Lack of acute xenogeneic graft- versus-host disease, but retention of T-cell function following engraftment of human peripheral blood mononuclear cells in NSG mice deficient in MHC class I and II expression.

ABSTRACT: Immunodeficient mice engrafted with human peripheral blood mononuclear cells (PBMCs) support preclinical studies of human pathogens, allograft rejection, and human T-cell function. However, a major limitation of PBMC engraftment is development of acute xenogeneic graft- versus-host disease (GVHD) due to human T-cell recognition of murine major histocompatibility complex (MHC). To address this, we created 2 NOD- scid IL-2 receptor subunit γ ( IL2rg) ^null (NSG) strains that lack murine MHC class I and II [NSG-β-2-microglobulin ( B2M) ^null ( IA IE)^null and NSG -( K^b D^b) ^null ( IA^null)]. We observed rapid human IgG clearance in NSG- B2M^null ( IA IE) ^null mice whereas clearance in NSG -( K^b</

SUBMITTER: Brehm MA 

PROVIDER: S-EPMC6404556 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature