Conditional Synaptic Vesicle Markers for Drosophila.

ABSTRACT: The release of neurotransmitters from synaptic vesicles (SVs) at pre-synaptic release sites is the principle means by which information transfer between neurons occurs. Knowledge of the location of SVs within a neuron can thus provide valuable clues about the location of neurotransmitter release within a neuron and the downstream neurons to which a given neuron is connected, important information for understanding how neural circuits generate behavior. Here the development and characterization of four conditional tagged SV markers for Drosophila melanogaster is presented. This characterization includes evaluation of conditionality, specificity for SV localization, and sensitivity of detection in diverse neuron subtypes. These four SV markers are genome-edited variants of the synapti

SUBMITTER: Williams JL 

PROVIDER: S-EPMC6404611 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature