BLASTing away preconceptions in crystallization trials.

ABSTRACT: Crystallization is in many cases a critical step for solving the three-dimensional structure of a protein molecule. Determining which set of chemicals to use in the initial screen is typically agnostic of the protein under investigation; however, crystallization efficiency could potentially be improved if this were not the case. Previous work has assumed that sequence similarity may provide useful information about appropriate crystallization cocktails; however, the authors are not aware of any quantitative verification of this assumption. This research investigates whether, given current information, one can detect any correlation between sequence similarity and crystallization cocktails. BLAST was used to quantitate the similarity between protein sequences in the Protein Data Bank, and t

SUBMITTER: Abrahams GJ 

PROVIDER: S-EPMC6404862 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature