Novel behavioral assays of spontaneous and precipitated THC withdrawal in mice.

ABSTRACT:

Background

A subset of cannabis users develop some degree of Cannabis Use Disorder (CUD). Although behavioral therapy has some success in treating CUD, many users relapse, often citing altered sleep, mood, and irritability. Preclinical animal tests of cannabinoid withdrawal focus primarily on somatic-related behaviors precipitated by a cannabinoid receptor antagonist. The goal of the present study was to develop novel cannabinoid withdrawal assays that are either antagonist-precipitated or spontaneously induced by abstinence.

Methods

C57BL/6 J mice were repeatedly administered the phytocannabinoid Δ⁹-tetrahydrocannabinol (THC; 1, 10 or 50 mg/kg, s.c.), the synthetic cannabinoid receptor agonist JWH-018 (1 mg/kg, s.c.), or vehicle (1:1:18 parts ethanol:Kolliphor EL

SUBMITTER: Trexler KR 

PROVIDER: S-EPMC6404969 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature