Integrated Functional Genomic Analysis Enables Annotation of Kidney Genome-Wide Association Study Loci.

ABSTRACT:

Background

Linking genetic risk loci identified by genome-wide association studies (GWAS) to their causal genes remains a major challenge. Disease-associated genetic variants are concentrated in regions containing regulatory DNA elements, such as promoters and enhancers. Although researchers have previously published DNA maps of these regulatory regions for kidney tubule cells and glomerular endothelial cells, maps for podocytes and mesangial cells have not been available.

Methods

We generated regulatory DNA maps (DNase-seq) and paired gene expression profiles (RNA-seq) from primary outgrowth cultures of human glomeruli that were composed mainly of podocytes and mesangial cells. We generated similar datasets from renal cortex cultures, to compare with those of the glomerular

SUBMITTER: Sieber KB 

PROVIDER: S-EPMC6405142 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature