T Cells Play a Causal Role in Diastolic Dysfunction during Uremic Cardiomyopathy.

ABSTRACT:

Background

Uremic cardiomyopathy, characterized by left ventricular hypertrophy, diastolic dysfunction, and impaired myocardial strain, contributes to increased cardiovascular mortality in patients with CKD. Emerging evidence suggests a pathogenic role for T cells during chronic heart failure.

Methods

To determine whether T cells contribute to uremic cardiomyopathy pathogenesis, we modeled this condition by inducing CKD via 5/6th nephrectomy in mice. We used flow cytometry to assess expression of markers of T cell memory or activation by lymphocytes from CKD mice and controls, as well as lymphocyte capacity for cytokine production. Flow cytometry was also used to quantify immune cells isolated from heart tissue. To test effects of T cell depletion on cardiac function,

SUBMITTER: Winterberg PD 

PROVIDER: S-EPMC6405145 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature