Nanobody immunostaining for correlated light and electron microscopy with preservation of ultrastructure.

ABSTRACT: Morphological and molecular characteristics determine the function of biological tissues. Attempts to combine immunofluorescence and electron microscopy invariably compromise the quality of the ultrastructure of tissue sections. We developed NATIVE, a correlated light and electron microscopy approach that preserves ultrastructure while showing the locations of multiple molecular moieties, even deep within tissues. This technique allowed the large-scale 3D reconstruction of a volume of mouse hippocampal CA3 tissue at nanometer resolution.

SUBMITTER: Fang T 

PROVIDER: S-EPMC6405223 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature