Cell-Free Expression and Photo-Crosslinking of the Human Neuropeptide Y2 Receptor.

ABSTRACT: G protein-coupled receptors (GPCRs) represent a large family of different proteins, which are involved in physiological processes throughout the entire body. Furthermore, they represent important drug targets. For rational drug design, it is important to get further insights into the binding mode of endogenous ligands as well as of therapeutic agents at the respective target receptors. However, structural investigations usually require homogenous, solubilized and functional receptors, which is still challenging. Cell-free expression methods have emerged in the last years and many different proteins are successfully expressed, including hydrophobic membrane proteins like GPCRs. In this work, an Escherichia coli based cell-free expression system was used to express the neuropeptide Y2 recept

SUBMITTER: Kogler LM 

PROVIDER: S-EPMC6405639 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature