Identification of CD4 and H-2K^d-restricted cytotoxic T lymphocyte epitopes on the human herpesvirus 6B glycoprotein Q1 protein.

ABSTRACT: The identification of Human herpesvirus 6B (HHV-6B) epitopes that are recognized by T-cells could contribute to the development of potential vaccines and immunotherapies. Here, we identified CD4⁺ and H-2K^d-restricted CD8⁺ T-cell epitopes on the glycoprotein Q1 of HHV-6B (BgQ1), which is a unique glycoprotein and essential for HHV-6B viral entry, by using in vivo electroporation with a plasmid DNA encoding BgQ1, overlapping peptides spanning the BgQ1 sequence, ELISPOT assay for quantification of gamma interferon (IFN-γ), and computer-based T-cell epitope prediction programs. The CD4⁺ and CD8⁺ T-cell epitopes identified in BALB/c mice in this study could be a good animal model system for use in the development of T-cell responses, induci

SUBMITTER: Nagamata S 

PROVIDER: S-EPMC6405833 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature