Functional characterization of iPSC-derived arterial- and venous-like endothelial cells.

ABSTRACT: The current work reports the functional characterization of human induced pluripotent stem cells (iPSCs)- arterial and venous-like endothelial cells (ECs), derived in chemically defined conditions, either in monoculture or seeded in a scaffold with mechanical properties similar to blood vessels. iPSC-derived arterial- and venous-like endothelial cells were obtained in two steps: differentiation of iPSCs into endothelial precursor cells (CD31^pos/KDR^pos/VE-Cad^med/EphB2^neg/COUP-TF^neg) followed by their differentiation into arterial and venous-like ECs using a high and low vascular endothelial growth factor (VEGF) concentration. Cells were characterized at gene, protein and functional levels. Functionally, both arterial and venous-like iPS

SUBMITTER: Rosa S 

PROVIDER: S-EPMC6405900 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature