MTrack: Automated Detection, Tracking, and Analysis of Dynamic Microtubules.

ABSTRACT: Microtubules are polar, dynamic filaments fundamental to many cellular processes. In vitro reconstitution approaches with purified tubulin are essential to elucidate different aspects of microtubule behavior. To date, deriving data from fluorescence microscopy images by manually creating and analyzing kymographs is still commonplace. Here, we present MTrack, implemented as a plug-in for the open-source platform Fiji, which automatically identifies and tracks dynamic microtubules with sub-pixel resolution using advanced objection recognition. MTrack provides automatic data interpretation yielding relevant parameters of microtubule dynamic instability together with population statistics. The application of our software produces unbiased and comparable quantitative datasets in a fully automat

SUBMITTER: Kapoor V 

PROVIDER: S-EPMC6405942 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature