Dental pulp cell-derived powerful inducer of TNF-α comprises PKR containing stress granule rich microvesicles.

ABSTRACT: It is well known that dental pulp tissue can evoke some of the most severe acute inflammation observed in the human body. We found that dental pulp cells secrete a factor that induces tumor necrosis factor-α production from macrophages, and designated this factor, dental pulp cell-derived powerful inducer of TNF-α (DPIT). DPIT was induced in dental pulp cells and transported to recipient cells via microvesicles. Treatment of dental pulp cells with a PKR inhibitor markedly suppressed DPIT activity, and weak interferon signals were constitutively activated inside the cells. In recipient macrophages, stimulation with DPIT-containing supernatants from pulp cells resulted in activation of both nuclear factor-κB and MAP kinases like JNK and p38. Proteomics analyses revealed that many stress gran

SUBMITTER: Suzuki S 

PROVIDER: S-EPMC6405945 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature