Indole Pulse Signalling Regulates the Cytoplasmic pH of E. coli in a Memory-Like Manner.

ABSTRACT: Bacterial cells are critically dependent upon pH regulation. Here we demonstrate that indole plays a critical role in the regulation of the cytoplasmic pH of Escherichia coli. Indole is an aromatic molecule with diverse signalling roles. Two modes of indole signalling have been described: persistent and pulse signalling. The latter is illustrated by the brief but intense elevation of intracellular indole during stationary phase entry. We show that under conditions permitting indole production, cells maintain their cytoplasmic pH at 7.2. In contrast, under conditions where no indole is produced, the cytoplasmic pH is near 7.8. We demonstrate that pH regulation results from pulse, rather than persistent, indole signalling. Furthermore, we illustrate that the relevant property of indole in th

SUBMITTER: Zarkan A 

PROVIDER: S-EPMC6405993 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature