RNA-Sequencing of Umbilical Cord Blood to Investigate Spontaneous Preterm Birth: A Pilot Study.

ABSTRACT: Objective  To analyze the transcriptomic gene expression of umbilical cord blood leukocytes using RNA-sequencing from preterm birth (PTB) and term birth (TB). Study Design  Eight women with spontaneous PTB (sPTB) and eight women with unlabored TB were enrolled prospectively. The sPTB and TB cohorts were matched for maternal age, race, mode of delivery, and fetal sex. Cord blood RNA was extracted and a globin depletion protocol was applied, then sequenced on the Illumina HiSeq 4000. Raw read counts were analyzed with DESeq2 to test for gene expression differences between sPTB and TB. Results  148 genes had significant differential expression ( q  < 0.01). Cell cycle/metabolism gene expression was significantly higher and immune/inflammatory signaling gene express

SUBMITTER: Vora NL 

PROVIDER: S-EPMC6406026 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature