High-Throughput Screening of Lipidomic Adaptations in Cultured Cells.

ABSTRACT: High-throughput screening of biologically active substances in cell cultures remains challenging despite great progress in contemporary lipidomic techniques. These experiments generate large amounts of data that are translated into lipid fingerprints. The subsequent visualization of lipidomic changes is key to meaningful interpretation of experimental results. As a demonstration of a rapid and versatile pipeline for lipidomic analysis, we cultured HeLa cells in 96-well format for four days in the presence or absence of various inhibitors of lipid metabolic pathways. Visualization of the data by principle component analysis revealed a high reproducibility of the method, as well as drug specific changes to the lipidome. Construction of heatmaps and networks revealed the similarities and diff

SUBMITTER: Jeucken A 

PROVIDER: S-EPMC6407004 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature