Transposon insertion profiling by sequencing (TIPseq) for mapping LINE-1 insertions in the human genome.

ABSTRACT:

Background

Transposable elements make up a significant portion of the human genome. Accurately locating these mobile DNAs is vital to understand their role as a source of structural variation and somatic mutation. To this end, laboratories have developed strategies to selectively amplify or otherwise enrich transposable element insertion sites in genomic DNA.

Results

Here we describe a technique, Transposon Insertion Profiling by sequencing (TIPseq), to map Long INterspersed Element 1 (LINE-1, L1) retrotransposon insertions in the human genome. This method uses vectorette PCR to amplify species-specific L1 (L1PA1) insertion sites followed by paired-end Illumina sequencing. In addition to providing a step-by-step molecular biology protocol, we offer users a guide to our pipel

SUBMITTER: Steranka JP 

PROVIDER: S-EPMC6407172 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature