Insulin action in the brain regulates mitochondrial stress responses and reduces diet-induced weight gain.

ABSTRACT:

Objective

Insulin action in the brain controls metabolism and brain function, which is linked to proper mitochondrial function. Conversely, brain insulin resistance associates with mitochondrial stress and metabolic and neurodegenerative diseases. In the present study, we aimed to decipher the impact of hypothalamic insulin action on mitochondrial stress responses, function and metabolism.

Methods

To investigate the crosstalk of insulin action and mitochondrial stress responses (MSR), namely the mitochondrial unfolded protein response (UPRmt) and integrated stress response (ISR), qPCR, western blotting, and mitochondrial activity assays were performed. These methods were used to analyze the hypothalamic cell line CLU183 treated with insulin in the presence or absence of the

SUBMITTER: Wardelmann K 

PROVIDER: S-EPMC6407370 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature