Time-lapse imaging of cell death in cell culture and whole living organisms using turn-on deep-red fluorescent probes.

ABSTRACT: Cell death is a central process in developmental biology and also an important indicator of disease status and treatment efficacy. Two related fluorescent probes are described that are molecular conjugates of one or two zinc dipicolylamine (ZnDPA) coordination complexes with an appended solvatochromic benzothiazolium squaraine dye. The probes were designed to target the anionic phospholipid, phosphatidylserine (PS), that is exposed on the surface of dead and dying cells. A series of spectrometric and microscopy studies using liposomes and red blood cell ghosts as models showed that the probe with two ZnDPA targeting units produced higher affinity, stronger fluorescence "turn-on" effect, and better image contrast than the probe with one ZnDPA. Both fluorescent probes enabled "no-wash" time-

SUBMITTER: Jarvis TS 

PROVIDER: S-EPMC6407891 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature