Membrane bridging by Munc13-1 is crucial for neurotransmitter release.

ABSTRACT: Munc13-1 plays a crucial role in neurotransmitter release. We recently proposed that the C-terminal region encompassing the C₁, C₂B, MUN and C₂C domains of Munc13-1 (C₁C₂BMUNC₂C) bridges the synaptic vesicle and plasma membranes through interactions involving the C₂C domain and the C₁-C₂B region. However, the physiological relevance of this model has not been demonstrated. Here we show that C₁C₂BMUNC₂C bridges membranes through opposite ends of its elongated structure. Mutations in putative membrane-binding sites of the C₂C domain disrupt the ability of C₁C₂BMUNC₂C to bridge liposomes and to mediate liposome fusion i

SUBMITTER: Quade B 

PROVIDER: S-EPMC6407922 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature