Regeneration of esophagus using a scaffold-free biomimetic structure created with bio-three-dimensional printing.

ABSTRACT: Various strategies have been attempted to replace esophageal defects with natural or artificial substitutes using tissue engineering. However, these methods have not yet reached clinical application because of the high risks related to their immunogenicity or insufficient biocompatibility. In this study, we developed a scaffold-free structure with a mixture of cell types using bio-three-dimensional (3D) printing technology and assessed its characteristics in vitro and in vivo after transplantation into rats. Normal human dermal fibroblasts, human esophageal smooth muscle cells, human bone marrow-derived mesenchymal stem cells, and human umbilical vein endothelial cells were purchased and used as a cell source. After the preparation of multicellular spheroids, esophageal-like tube structure

SUBMITTER: Takeoka Y 

PROVIDER: S-EPMC6408002 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature