Extensive Heterogeneity and Intrinsic Variation in Spatial Genome Organization.

ABSTRACT: Several general principles of global 3D genome organization have recently been established, including non-random positioning of chromosomes and genes in the cell nucleus, distinct chromatin compartments, and topologically associating domains (TADs). However, the extent and nature of cell-to-cell and cell-intrinsic variability in genome architecture are still poorly characterized. Here, we systematically probe heterogeneity in genome organization. High-throughput optical mapping of several hundred intra-chromosomal interactions in individual human fibroblasts demonstrates low association frequencies, which are determined by genomic distance, higher-order chromatin architecture, and chromatin environment. The structure of TADs is variable between individual cells, and inter-TAD associations

SUBMITTER: Finn EH 

PROVIDER: S-EPMC6408223 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature