PNPLA3 Gene Polymorphisms in HCV/HIV-Coinfected Individuals.

ABSTRACT:

Background and aims

The patatin-like phospholipase domain-containing 3 (PNPLA3) gene has been associated with the development of alcoholic and nonalcoholic steatohepatitis. Using a newly developed and validated assay for PNPLA3, we explored the prevalence of gene polymorphisms in a cohort of HCV/HIV-coinfected individuals to determine whether there was an association with insulin resistance or hepatic fibrosis.

Methods

A high-resolution melting point (HRM) assay was developed and validated. The assay was used to evaluate samples obtained in the context of a clinical trial performed at ACTG sites across the USA in HIV-infected patients. Clinical features and treatment outcomes were assessed in relation to the PNPLA3 genotype.

Results

The HRM methodology demonstrated 10

SUBMITTER: Sherman KE 

PROVIDER: S-EPMC6408225 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature