A Ca²⁺-regulated deAMPylation switch in human and bacterial FIC proteins.

ABSTRACT: FIC proteins regulate molecular processes from bacteria to humans by catalyzing post-translational modifications (PTM), the most frequent being the addition of AMP or AMPylation. In many AMPylating FIC proteins, a structurally conserved glutamate represses AMPylation and, in mammalian FICD, also supports deAMPylation of BiP/GRP78, a key chaperone of the unfolded protein response. Currently, a direct signal regulating these FIC proteins has not been identified. Here, we use X-ray crystallography and in vitro PTM assays to address this question. We discover that Enterococcus faecalis FIC (EfFIC) catalyzes both AMPylation and deAMPylation and that the glutamate implements a multi-position metal switch whereby Mg²⁺ and Ca²⁺ control AMPylation and deAMPylation differential

SUBMITTER: Veyron S 

PROVIDER: S-EPMC6408439 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature