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Correcting locomotion dependent observation biases in thermal preference of Drosophila.


ABSTRACT: Sensing environmental temperatures is essential for the survival of ectothermic organisms. In Drosophila, two of the most used methodologies to study temperature preferences (TP) and the genes involved in thermosensation are two-choice assays and temperature gradients. Whereas two-choice assays reveal a relative TP, temperature gradients can identify the absolute Tp. One drawback of gradients is that small ectothermic animals are susceptible to cold-trapping: a physiological inability to move at the cold area of the gradient. Often cold-trapping cannot be avoided, biasing the resulting TP to lower temperatures. Two mathematical models were previously developed to correct for cold-trapping. These models, however, focus on group behaviour which can

SUBMITTER: Giraldo D 

PROVIDER: S-EPMC6408449 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature