RNA Stabilizes Transcription-Dependent Chromatin Loops Induced By Nuclear Hormones.

ABSTRACT: We show that transcription induced by nuclear receptors for estrogen (E₂) or retinoic acid (RA) is associated with formation of chromatin loops that juxtapose the 5' end (containing the promoter) with the enhancer and the 3' polyA addition site of the target gene. We find three loop configurations which change as a function of time after induction: 1. RA or E₂-induced loops which connect the 5' end, the enhancer and the 3' end of the gene, and are stabilized by RNA early after induction; 2. E₂-independent loops whose stability does not require RNA; 3. Loops detected only by treatment of chromatin with RNAse H1 prior to hormonal induction. RNAse H1 digests RNA that occludes the relevant restriction sites, thus preventing detection of these loops. R-loops at

SUBMITTER: Pezone A 

PROVIDER: S-EPMC6408484 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature