E-syt1 Re-arranges STIM1 Clusters to Stabilize Ring-shaped ER-PM Contact Sites and Accelerate Ca²⁺ Store Replenishment.

ABSTRACT: In many non-excitable cells, the depletion of endoplasmic reticulum (ER) Ca²⁺ stores leads to the dynamic formation of membrane contact sites (MCSs) between the ER and the plasma membrane (PM), which activates the store-operated Ca²⁺ entry (SOCE) to refill the ER store. Two different Ca²⁺-sensitive proteins, STIM1 and extended synaptotagmin-1 (E-syt1), are activated during this process. Due to the lack of live cell super-resolution imaging, how MCSs are dynamically regulated by STIM1 and E-syt1 coordinately during ER Ca²⁺ store depletion and replenishment remain unknown. With home-built super-resolution microscopes that provide superior axial and lateral resolution in live cells, we revealed that extracellular Ca²⁺ influx via SOCE acti

SUBMITTER: Kang F 

PROVIDER: S-EPMC6408583 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature