Chromosome screening using culture medium of embryos fertilised in vitro: a pilot clinical study.

ABSTRACT:

Background

Previous studies from this as well as other research groups suggested that non-invasive chromosome screening (NICS) with embryo culture medium can be used to identify chromosomal ploidy and chromosomal abnormalities. We here report a series of clinical cases utilizing the technology.

Methods

A total of 45 couples underwent in vitro fertilisation during a period between February 2016 and February 2017. Karyotyping revealed normal chromosomes in both partners in 23 couples, and chromosomal rearrangements in at least one partner in 22 couples. Intracytoplasmic sperm injection (ICSI) was used for fertilization. NICS was carried out using embryo culture medium at the blastocyst stage via multiple annealing and looping-based amplification cycles, whole-genome amplificat

SUBMITTER: Fang R 

PROVIDER: S-EPMC6408780 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature