Multicolour three dimensional structured illumination microscopy of immunolabeled plant microtubules and associated proteins.

ABSTRACT:

Background

In the present work, we provide an account of structured illumination microscopy (SIM) imaging of fixed and immunolabeled plant probes. We take advantage of SIM, to superresolve intracellular structures at a considerable z-range and circumvent its low temporal resolution capacity during the study of living samples. Further, we validate the protocol for the imaging of fixed transgenic material expressing fluorescent protein-based markers of different subcellular structures.

Results

Focus is given on 3D imaging of bulky subcellular structures, such as mitotic and cytokinetic microtubule arrays as well as on the performance of SIM using multichannel imaging and the quantitative correlations that can be deduced. As a proof of concept, we provide a superresolution outp

SUBMITTER: Vavrdova T 

PROVIDER: S-EPMC6408805 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature