Development of a Transformation Method for Metschnikowia borealis and other CUG-Serine Yeasts.

ABSTRACT: Yeasts belonging to the Metschnikowia genus are particularly interesting for the unusual formation of only two needle-shaped ascospores during their mating cycle. Presently, the meiotic process that can lead to only two spores from a diploid zygote is poorly understood. The expression of fluorescent nuclear proteins should allow the meiotic process to be visualized in vivo; however, no large-spored species of Metschnikowia has ever been transformed. Accordingly, we aimed to develop a transformation method for Metschnikowiaborealis, a particularly large-spored species of Metschnikowia, with the goal of enabling the genetic manipulations required to study biological processes in detail. Genetic analyses confirmed that M. borealis, and many other M

SUBMITTER: Gordon ZB 

PROVIDER: S-EPMC6409616 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature