Characteristics of the First Protein Tyrosine Phosphatase with Phytase Activity from a Soil Metagenome.

ABSTRACT: Protein tyrosine phosphatases (PTPs) fulfil multiple key regulatory functions. Within the group of PTPs, the atypical lipid phosphatases (ALPs) are known for their role as virulence factors associated with human pathogens. Another group of PTPs, which is capable of using inositol-hexakisphosphate (InsP₆) as substrate, are known as phytases. Phytases play major roles in the environmental phosphorus cycle, biotechnology, and pathogenesis. So far, all functionally characterized PTPs, including ALPs and PTP-phytases, have been derived exclusively from isolated microorganisms. In this study, screening of a soil-derived metagenomic library resulted in identification of a gene (pho16B), encoding a PTP, which shares structural characteristics with the ALPs. In addition, the characterization

SUBMITTER: Castillo Villamizar GA 

PROVIDER: S-EPMC6409689 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature