SCRaMbLE generates evolved yeasts with increased alkali tolerance.

ABSTRACT:

Background

Strains with increased alkali tolerance have a broad application in industrial, especially for bioremediation, biodegradation, biocontrol and production of bio-based chemicals. A novel synthetic chromosome recombination and modification by LoxP-mediated evolution (SCRaMbLE) system has been introduced in the synthetic yeast genome (Sc 2.0), which enables generation of a yeast library with massive structural variations and potentially drives phenotypic evolution. The structural variations including deletion, inversion and duplication have been detected within synthetic yeast chromosomes.

Results

Haploid yeast strains harboring either one (synV) or two (synV and synX) synthetic chromosomes were subjected to SCRaMbLE. Seven of evolved strains with increased alkali tol

SUBMITTER: Ma L 

PROVIDER: S-EPMC6410612 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature