IL-23 and IL-17A are not involved in hepatic/ischemia reperfusion injury in mouse and man.

ABSTRACT:

Background

Hepatic ischemia and reperfusion (I/R) is common in liver surgery and transplantation and compromises postoperative liver function. Hepatic I/R injury is characterized by sterile inflammation that contributes to hepatocellular necrosis. Many immune cells and cytokines have been implicated in hepatic I/R injury. However, the role and relevance of IL-23 and IL-17A remains controversial in literature. Aim: To determine whether the IL-23/IL-17A signaling axis is activated in hepatic I/R using a triple-level experimental approach (in vitro, in vivo, and clinical).

Methods

IL-23 and IL-17A were assayed by ELISA in the supernatant fractions of cultured murine (RAW 264.7) macrophages that were activated by supernatant fractions of necrotic cultured mouse (AML12) he

SUBMITTER: Olthof PB 

PROVIDER: S-EPMC6410619 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature