Comparison of K-Ras and N-Ras Mutagenic Hot Spots for UVC Damage.

ABSTRACT: It has been well established that mutations in K-Ras and N-Ras proto-oncogenes can convert them into active oncogenes. Current molecular cancer research has been focused on determining the key steps by which cellular genes become oncogenes and not on the underlying and fundamental chemical damage mechanism and susceptibility to damage. In this study, we investigate the damage hot spots present in the N-Ras and K-Ras genes upon exposure to UVC radiation. Detection of damage is accomplished by a simple, sensitive, mix-and-read assay using an EvaGreen probe in a 96-well microtiter plate. Our results show that, although there is high degree of sequential similarities among K-Ras and N-Ras genes, they show different degrees of UV damage in different por

SUBMITTER: Nair SG 

PROVIDER: S-EPMC6410678 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature