Live imaging reveals hub cell assembly and compaction dynamics during morphogenesis of the Drosophila testis niche.

ABSTRACT: Adult stem cells are often found in specialized niches, where the constituent cells direct self-renewal of their stem cell pool. The niche is therefore crucial for both normal homeostasis and tissue regeneration. In many mammalian tissues, niche cells have classically been difficult to identify, which has hampered any understanding of how tissues first construct niches during development. Fortunately, the Drosophila germline stem cell (GSC) niche is well defined, allowing for unambiguous identification of both niche cells and resident stem cells. The testis niche first forms in the early embryo, during a late stage of gonadogenesis. Here, using live-imaging both in vivo and ex vivo, we follow pro-niche cells as they assemble and assume their final form. We show that after ex vivo culture t

SUBMITTER: Anllo L 

PROVIDER: S-EPMC6410746 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature