Early onset preeclampsia in a model for human placental trophoblast.

ABSTRACT: We describe a model for early onset preeclampsia (EOPE) that uses induced pluripotent stem cells (iPSCs) generated from umbilical cords of EOPE and control (CTL) pregnancies. These iPSCs were then converted to placental trophoblast (TB) representative of early pregnancy. Marker gene analysis indicated that both sets of cells differentiated at comparable rates. The cells were tested for parameters disturbed in EOPE, including invasive potential. Under 5% O₂, CTL TB and EOPE TB lines did not differ, but, under hyperoxia (20% O₂), invasiveness of EOPE TB was reduced. RNA sequencing analysis disclosed no consistent differences in expression of individual genes between EOPE TB and CTL TB under 20% O₂, but, a weighted correlation network analysis revealed two gen

SUBMITTER: Sheridan MA 

PROVIDER: S-EPMC6410818 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature