Pluripotent stem cell-derived myogenic progenitors remodel their molecular signature upon in vivo engraftment.

ABSTRACT: Optimal cell-based therapies for the treatment of muscle degenerative disorders should not only regenerate fibers but provide a quiescent satellite cell pool ensuring long-term maintenance and regeneration. Conditional expression of Pax3/Pax7 in differentiating pluripotent stem cells (PSCs) allows the generation of myogenic progenitors endowed with enhanced regenerative capacity. To identify the molecular determinants underlying their regenerative potential, we performed transcriptome analyses of these cells along with primary myogenic cells from several developmental stages. Here we show that in vitro-generated PSC-derived myogenic progenitors possess a molecular signature similar to embryonic/fetal myoblasts. However, compared with fetal myoblasts, following transplantation they show sup

SUBMITTER: Incitti T 

PROVIDER: S-EPMC6410870 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature