Caprine humoral response to Burkholderia pseudomallei antigens during acute melioidosis from aerosol exposure.

ABSTRACT: Burkholderia pseudomallei causes melioidosis, a common source of pneumonia and sepsis in Southeast Asia and Northern Australia that results in high mortality rates. A caprine melioidosis model of aerosol infection that leads to a systemic infection has the potential to characterize the humoral immune response. This could help identify immunogenic proteins for new diagnostics and vaccine candidates. Outbred goats may more accurately mimic human infection, in contrast to the inbred mouse models used to date. B. pseudomallei infection was delivered as an intratracheal aerosol. Antigenic protein profiling was generated from the infecting strain MSHR511. Humoral immune responses were analyzed by ELISA and western blot, and the antigenic proteins were identified by mass spectrometry. Throughout

SUBMITTER: Yi J 

PROVIDER: S-EPMC6411198 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature