Adult human pancreatic acinar cells dedifferentiate into an embryonic progenitor-like state in 3D suspension culture.

ABSTRACT: Human pancreatic exocrine cells were cultured in 3D suspension and formed pancreatospheres composed of acinar-derived and duct-like cells. We investigated, up to 6 days, the fate of human pancreatic acinar cells using fluorescein-conjugated Ulex Europaeus Agglutinin 1 lectin, a previously published acinar-specific non-genetic lineage tracing strategy. At day 4, fluorescence-activated cell sort for the intracellularly incorporated FITC-conjugated UEA1 lectin and the duct-specific CA19.9 surface marker, distinguished acinar-derived cells (UEA1⁺CA19.9^-) from duct-like cells (UEA1^-CA19.9⁺) and acinar-to-duct-like transdifferentiated cells (UEA1⁺CA19.9⁺). mRNA expression analysis of the acinar-derived (UEA1⁺CA19.9

SUBMITTER: Baldan J 

PROVIDER: S-EPMC6411888 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature