Optimization of ribosome profiling using low-input brain tissue from fragile X syndrome model mice.

ABSTRACT: Dysregulated protein synthesis is a major underlying cause of many neurodevelopmental diseases including fragile X syndrome. In order to capture subtle but biologically significant differences in translation in these disorders, a robust technique is required. One powerful tool to study translational control is ribosome profiling, which is based on deep sequencing of mRNA fragments protected from ribonuclease (RNase) digestion by ribosomes. However, this approach has been mainly applied to rapidly dividing cells where translation is active and large amounts of starting material are readily available. The application of ribosome profiling to low-input brain tissue where translation is modest and gene expression changes between genotypes are expected to be small has not been carefully evaluat

SUBMITTER: Liu B 

PROVIDER: S-EPMC6411937 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature