An Exome-seq Based Tool for Mapping and Selection of Candidate Genes in Maize Deletion Mutants.

ABSTRACT: Despite the large number of genomic and transcriptomic resources in maize, there is still much to learn about the function of genes in developmental and biochemical processes. Some maize mutants that were generated by gamma-irradiation showed clear segregation for the kernel phenotypes in B73 × Mo17 F2 ears. To better understand the functional genomics of kernel development, we developed a mapping and gene identification pipeline, bulked segregant exome sequencing (BSEx-seq), to map mutants with kernel phenotypes including opaque endosperm and reduced kernel size. BSEx-seq generates and compares the sequence of the exon fraction from mutant and normal plant F2 DNA pools. The comparison can derive mapping peaks, identify deletions within the mapping peak, and suggest candidate genes within

SUBMITTER: Jia S 

PROVIDER: S-EPMC6411947 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature