Bayesian inference of origin firing time distributions, origin interference and licencing probabilities from Next Generation Sequencing data.

ABSTRACT: DNA replication is a stochastic process with replication forks emanating from multiple replication origins. The origins must be licenced in G1, and the replisome activated at licenced origins in order to generate bi-directional replication forks in S-phase. Differential firing times lead to origin interference, where a replication fork from an origin can replicate through and inactivate neighbouring origins (origin obscuring). We developed a Bayesian algorithm to characterize origin firing statistics from Okazaki fragment (OF) sequencing data. Our algorithm infers the distributions of firing times and the licencing probabilities for three consecutive origins. We demonstrate that our algorithm can distinguish partial origin licencing and origin obscuring in OF sequencing data from Saccharom

SUBMITTER: Bazarova A 

PROVIDER: S-EPMC6412128 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature