Optimisation of Protein Extraction from Medicinal Cannabis Mature Buds for Bottom-Up Proteomics.

ABSTRACT: Medicinal cannabis is used to relieve the symptoms of certain medical conditions, such as epilepsy. Cannabis is a controlled substance and until recently was illegal in many jurisdictions. Consequently, the study of this plant has been restricted. Proteomics studies on Cannabis sativa reported so far have been primarily based on plant organs and tissues other than buds, such as roots, hypocotyl, leaves, hempseeds and flour. As far as we know, no optimisation of protein extraction from cannabis reproductive tissues has been attempted. Therefore, we set out to assess different protein extraction methods followed by mass spectrometry-based proteomics to recover, separate and identify the proteins of the reproductive organs of medicinal cannabis, apical buds and isolated trichomes. Data

SUBMITTER: Vincent D 

PROVIDER: S-EPMC6412734 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature