Multinucleation of Incubated Cells and Their Morphological Differences Compared to Mononuclear Cells.

ABSTRACT: Some cells cultured in vitro have multiple nuclei. Since cultured cells are used in various fields of science, including tissue engineering, the nature of the multinucleated cells must be determined. However, multinucleated cells are not frequently observed. In this study, a method to efficiently obtain multinucleated cells was established and their morphological properties were investigated. Initially, we established conditions to quickly and easily generate multinucleated cells by seeding a Xenopus tadpole epithelium tissue-derived cell line (XTC-YF) on less and more hydrophilic dishes, and incubating the cultures with medium supplemented with or without Y-27632-a ROCK inhibitor-to reduce cell contractility. Notably, 88% of the cells cultured on a less hydrophilic dish in medium suppleme

SUBMITTER: Sugita S 

PROVIDER: S-EPMC6412785 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature