Ubiquinol-cytochrome C reductase core protein II promotes tumorigenesis by facilitating p53 degradation.

ABSTRACT:

Background

Ubiquinol-cytochrome C reductase core protein II (QCR2) is essential for mitochondrial functions, yet, its role in cancer development has remained elusive.

Methods

The expression of QCR2 in cancer patients was assessed by immunohistochemistry. The proliferation of cancer cells was assessed by CCK-8 assay, EdU staining and Flow cytometry analysis. The biological function of QCR2 and PHB were determined using western blotting, RT-qPCR, microarray analysis and xenografts. The interactions between proteins and the ubiquitination of p53 were assessed by immunoprecipitation, mass spectrometry analysis and GST pull down. The subcellular location of PHB and QCR2 was assessed by immunoblotting and immunofluorescence.

Finding

The expression of QCR2 is upregulated in

SUBMITTER: Han Y 

PROVIDER: S-EPMC6412871 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature