Endogenous Gene Regulation as a Predicted Main Function of Type I-E CRISPR/Cas System in E. coli.

ABSTRACT: CRISPR/Cas is an adaptive bacterial immune system, whose CRISPR array can actively change in response to viral infections. However, Type I-E CRISPR/Cas in E. coli (an established model system), appears not to exhibit such active adaptation, which suggests that it might have functions other than immune response. Through computational analysis, we address the involvement of the system in non-canonical functions. To assess targets of CRISPR spacers, we align them against both E. coli genome and an exhaustive (~230) set of E. coli viruses. We systematically investigate the obtained alignments, such as hit distribution with respect to genome annotation, propensity to target mRNA, the target functional enrichment, conservation of CRISPR spacers and putative targets in relate

SUBMITTER: Bozic B 

PROVIDER: S-EPMC6413058 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature