Quality Screening of Incorrectly Folded Soluble Aggregates from Functional Recombinant Proteins.

ABSTRACT: Solubility is the prime criterion for determining the quality of recombinant proteins, yet it often fails to represent functional activity due to the involvement of non-functional, misfolded, soluble aggregates, which compromise the quality of recombinant proteins. However, guidelines for the quality assessment of soluble proteins have neither been proposed nor rigorously validated experimentally. Using the aggregation-prone enhanced green-fluorescent protein (EGFP) folding reporter system, we evaluated the folding status of recombinant proteins by employing the commonly used sonication and mild lysis of recombinant host cells. We showed that the differential screening of solubility and folding competence is crucial for improving the quality of recombinant proteins without sacrificing thei

SUBMITTER: Kwon SB 

PROVIDER: S-EPMC6413200 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature