RFX1 downregulation contributes to TLR4 overexpression in CD14⁺ monocytes via epigenetic mechanisms in coronary artery disease.

ABSTRACT:

Background

Toll-like receptor 4 (TLR4) expression is increased in activated monocytes, which play a critical role in the pathogenesis of coronary artery disease (CAD). However, the mechanism remains unclear. Regulatory factor X1 (RFX1) is a critical transcription factor regulating epigenetic modifications. In this study, we investigated whether RFX1 and epigenetic modifications mediated by RFX1 contributed to the overexpression of TLR4 in activated monocytes.

Results

Compared with those of the controls, the mRNA and protein expression of RFX1 were downregulated and the mRNA expression of TLR4 was upregulated in CD14⁺ monocytes obtained from CAD patients and CD14⁺ monocytes obtained from healthy controls treated with low-density lipoprotein (LDL). The mR

SUBMITTER: Du P 

PROVIDER: S-EPMC6413463 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature