A core-weighted fitting method for docking atomic structures into low-resolution maps: application to cryo-electron microscopy.

ABSTRACT: Cryo-electron microscopy of "single particles" is a powerful method to analyze structures of large macromolecular assemblies that are not amenable to investigation by traditional X-ray crystallographic methods. A key step in these studies is to obtain atomic interpretations of multiprotein complexes by fitting atomic structures of individual components into maps obtained from electron microscopic data. Here, we report the use of a "core-weighting" method, combined with a grid-threading Monte Carlo (GTMC) approach for this purpose. The "core" of an individual structure is defined to represent the part where the density distribution is least likely to be altered by other components that comprise the macromolecular assembly of interest. The performance of the method has been evaluated by its

SUBMITTER: Wu X 

PROVIDER: S-EPMC6413516 | biostudies-literature | 2003 Jan

REPOSITORIES: biostudies-literature