LRH1-driven transcription factor circuitry for hepatocyte identity: Super-enhancer cistromic analysis.

ABSTRACT:

Background

The injured liver loses normal function, with concomitant decrease of key identity genes. Super-enhancers contribute to mammalian cell identity. Here, we identified core transcription factors (TFs) that are active in hepatocytes, using genome-wide analysis and hierarchical ordering of super-enhancer distribution.

Methods

Expression of core TFs was assessed in a cohort of patients with hepatitis or cirrhosis and animal models. Quantitative PCR, chromatin immunoprecipitation assays, and hydrodynamic gene delivery methods were used to assess gene regulation and hepatocyte viability. RNA-sequencing data were generated to investigate the role of LRH1 in hepatocyte protection from injury.

Results

Network analysis of super-enhancer-associated gene interactions and

SUBMITTER: Joo MS 

PROVIDER: S-EPMC6413675 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature