Combining 3D graphene-like screen-printed carbon electrode with methylene blue-loaded liposomal nanoprobes for phospholipase A₂ detection.

ABSTRACT: Phospholipase A₂ (PLA₂) enzyme could be acted as a unique biomarker for forecasting and diagnosing certain diseases. Therefore, it is important to monitor PLA₂ activity in biological and clinical samples. In this work, a simple electrochemical assay for PLA₂ activity was developed based on a screen-printed carbon electrode (SPCE) with 3D graphene-like surface. When the PLA₂-containing sample was mixed with the nanoprobes, i.e. the electroactive marker methylene blue (MB) encapsulated within nanometer-sized phospholipid liposomes, MB was released and adsorbed/enriched in site onto the surface of SPCE in a micro-cell. The encapsulation and enzymatic release of MB were evaluated using UV-Vis and fluorescence. The peak current due to oxida

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC6413735 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature